School of Basic and Applied Science

Department of Biochemistry and Microbial Sciences


The Centre for Biochemistry and Microbial Sciences within the School of Basic and Applied Sciences started as an independent center in 2015 with an aim to train the students in the fields of biochemistry and microbiology and to encourage interdisciplinary activities among the faculty. The Centre has internationally trained faculty with wide research and teaching experience. The Centre is currently engaged in active research and with several research projects funded by various funding agencies such as DST and DBT. The centre strives to synergize the study of biochemistry and microbiology with health disparities research through innovation and collaboration and to provide the highest quality of translational biomedical research, education, and service. The internationally trained faculty of the centre is committed to provide outstanding teaching in the biochemical and microbial sciences and to conduct quality research of international repute and promote R&D activities in the emerging areas of biochemistry and microbiology. The centre is involved in the community service and awareness programs related to medical biochemistry and microbiology. The focus of the centre is to:

  • Provide state of the art educational and research training for students.
  • Provide students excellent opportunities to explore the dynamic fields of biomedical including clinical biochemistry, immune-pharmacology, immunology, and drug research.

Research Thrust Areas

  1. Cancer Biology
  2. Plant Natural Products
  3. Immunology of Clinically Relevant Diseases
  4. Systems Biology
  5. Host Microbe Interactions

Faculty

  1. Dr. Ramakrishna Wusirika, Professor, Centre Coordinator
  2. Dr. Monisha Dhiman, Associate Professor
  3. Dr. Malkhey Verma, Associate Professor
  4. Dr. Shashank Kumar, Assistant Professor
  5. Dr. Somesh Baranwal, Assistant Professor
  6. Dr. Pramod Kushawaha, Assistant Professor
  7. Dr. Manju Jain,Assistant Professor

Academic Programmes Offered

  1. Ph.D. Biochemistry
  2. Ph.D. Microbial Sciences
  3. M. Sc. Life Sciences with specialization in Biochemistry
  4. M. Sc. Life Sciences with specialization in Microbial Sciences

Syllabi

2017-18

2016-17

2015-16

Research Highlights/ Areas

  • Molecular and Cellular Biology of Gastro-intestinal Cancer
  • Development of novel plant natural products as anti-cancer drugs
  • Synthetic System Biology and Bioprocess Engineering
  • Role of metal resistant bacteria and mycorrhiza in enhancing plant growth and metal uptake by modulating metabolic pathways
  • Chemotherapy induced toxicity
  • Signaling pathways during Inflammation and oxidative stress in various human diseases
  • Drug and Vaccine development to Visceral leishmaniasis (Kalazar)
  • Understanding immuno-biology of clinically relevant diseases associated with an abnormal T cell effector immune response

Laboratory Facilities

The centre has the following equipment:
  • Centrifuge
  • Microscopes
  • Water Bath
  • Refrigerators
  • Laminar air flow cabinet
  • PCR machine
  • Mini sub cell GT – horizontal gel electrophoresis unit
  • Rotamantle
  • BOD incubator
  • Incubator shaker
  • Autoclaves
  • Spectrophotometer
  • Digital analytical balance

The centre has access to well-equipped teaching and research laboratories with the following equipment:

  • Ultra-low temperature freezers
  • Cell culture facility
  • CO2 incubators
  • Laminar air flow cabinets
  • Real-time PCR
  • DNA sequencer
  • Gel documentation system

The centre also has access to the following equipment in the Central Instrumentation Laboratory (CIL):

  • Confocal Microscope
  • Scanning Electron Microscope
  • GC-MS
  • UHPLC
  • Flow Cytometer
  • Atomic Absorption Spectrometer
  • Fourier Transform Infrared Spectrometer
  • ICP-MS

Publication

Dr. Ramakrishna Wusirika, Professor, Centre Coordinator
Peer-reviewed Research Publications

  • Dhawi F, Datta R, Ramakrishna W (2017) Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1865 (2): 243-251. Impact Factor 2.9
  • Rahman N, Dhadi SR, Deshpande A, Ramakrishna W (2016) Rice callus suspension culture inhibits growth of cell lines of multiple cancer types and induces apoptosis in lung cancer cell line. BMC Complementary and Alternative Medicine 16 (1), 427. Impact Factor 2.3
  • Dhawi F, Datta R, Ramakrishna W (2016) Mycorrhiza and heavy metal resistant bacteria enhance growth, nutrient uptake and alter metabolic profile of sorghum grown in marginal soil. Chemosphere 157, 33-41. Impact Factor 4.5
  • Pidatala VR, Li K, Sarkar D, Ramakrishna W, Datta R. (2016) Identification of biochemical pathways associated with lead tolerance and detoxification in Chrysopogon zizanioides L. Nash (Vetiver) by metabolic profiling. Environmental Science and Technology 50:2530-2537. Impact Factor 6.2
  • Dhawi F, Datta R, Ramakrishna W. (2015) Mycorrhiza and PGPB modulate maize biomass, nutrient uptake and metabolic pathways in maize grown in mining-impacted soil. Plant Physiology and Biochemistry 97:390-399. Impact Factor 3.3
  • Dhadi SR, Xu Z, Shaik R,  Driscoll K, Ramakrishna W. (2015)  Differential regulation of genes by retrotransposons in rice promoters. Plant Molecular Biology 87:603-613. Impact Factor 4.3
  • Li K, Pidatala VR, Shaik R, Datta R, Ramakrishna W. (2014) Integrated metabolomic and proteomic approaches dissect the effect of metal resistant bacteria on maize biomass and copper uptake.  Environmental Science and Technology 48:1184–1193. Impact Factor 5.3
  • Shaik R, Ramakrishna W.  (2014) Machine learning approaches distinguish multiple stress conditions using stress-responsive genes and identify candidate genes for broad resistance in rice. Plant Physiology 164:481-495. Impact Factor 8
  • Shaik R, Ramakrishna W. (2013) Genes and coexpression modules common to drought and bacterial stress responses in Arabidopsis and rice.  PloS One 8:e77261. Impact Factor 3.2
  • Dhadi SR, Deshpande A, Driscoll K, Ramakrishna W. (2013) Major cis-regulatory elements for rice bidirectional promoter activity reside in the 5’-untranslated regions. Gene 526:400–410. Impact Factor 2.1
  • Rafi S, Ramakrishna W.  (2012) Bioinformatic analysis of epigenetic and microRNA mediated regulation of drought responsive genes in rice. PloS One 7:e49331. Impact Factor 3.2
  • Li K, Pidatala RR, Ramakrishna W.  (2012) Mutational, proteomic and metabolomic analysis of a plant growth promoting copper-resistant Pseudomonas spp. FEMS Microbiology Letters 335:140–148. Impact Factor 2.1
  • Deshpande AD, Dhadi SR, Hager E, Ramakrishna W.  (2012) Rice callus suspension cultures inhibit growth of two cancer cell lines. Phytotherapy Research 26:1075–1081. Impact Factor 2.7
  • Krom N, Ramakrishna, W. (2012) Retrotransposon insertions in rice gene pairs associated with reduced conservation of gene pairs in grass genomes. Genomics 99:308-14. Impact Factor 3.3
  • Dhadi SR, Deshpande A, Ramakrishna W. (2012) A novel nonwounding transient expression assay for cereals mediated by Agrobacterium tumefaciens.  Plant Molecular Biology Reporter 30:36-45. Impact Factor 1.7
  • Li K, Ramakrishna W. (2011) Effect of multiple metal resistant bacteria from contaminated lake sediments on metal accumulation and plant growth. Journal of Hazardous Materials 189:531-539. Impact Factor 4.5
  • Xu Z, Rafi S, Ramakrishna W. (2011) Polymorphisms and evolutionary history of retrotransposon insertions in rice promoters.  Genome 54:629-638. Impact Factor 1.7
  • Krom N, Ramakrishna W. (2011) Conservation, rearrangement, and deletion of gene pairs during the evolution of four grass genomes.  DNA Research 17:343-352. Impact Factor 5.5
  • Dhadi SR, Krom N, Ramakrishna W. (2009) Genome-wide comparative analysis of putative bidirectional promoters from rice, Arabidopsis, and Populus.  Gene 429:65-73.  Impact Factor 2.1
  • Krom, N, Ramakrishna W. (2008) Comparative analysis of divergent and convergent gene pairs and their expression patterns in rice, Arabidopsis, and PopulusPlant Physiology  147:1763-1773. Impact Factor 8
  • Krom, N, Recla J, Ramakrishna W. (2008) Analysis of genes associated with retrotransposons in the rice genome.  Genetica 134:297-310. Impact Factor 2.2
  • Xu Z, Ramakrishna W.  (2008)Retrotransposon insertion polymorphisms in six rice genes and their evolutionary history.  Gene 412:50-58.  Impact Factor 2.1
  • Oakley RV, Wang Y-S, Ramakrishna W, Harding SA, Tsai CJ. (2007) Differential expansion and expression of α- and β-tubulin gene families in Populus.  Plant Physiology 145:961-973. Impact Factor 8
  • Nagy E, Lee T-C, Ramakrishna W, Xu Z, Klein P, SanMiguel P, Cheng C-P, Li J, Devos K, Schertz K, Dunkle L, Bennetzen J.  (2007) Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin. Theoretical Applied Genetics 114:961-970. Impact Factor 3.8
  • Gorantla M, Babu P, Reddy Lachagari V, Reddy A, Wusirika R, Bennetzen JL, Reddy AR.  (2007) Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings. Journal of Experimental Botany 58:253-265. Impact Factor 5.5
  • Lai J, Ma J, Swigonova Z, Ramakrishna W, Linton E, Llaca V, Tanyolac B, Park YJ, Jeong OY, Bennetzen JL, Messing J (2004) Gene loss and movement in the maize genome.  Genome Research 14:1924-1931.  Impact Factor 13.9
  • Swigonova Z, Lai J, Ma J, Ramakrishna W, Llaca V, Bennetzen JL, Messing J. (2004) Close split of sorghum and maize genome progenitors.  Genome Research 14:1916-1923. Impact Factor 13.9
  • Yan L, Loukoianov A, Blechl A, Tranquilli G, Ramakrishna W, SanMiguel P, Bennetzen JL, Echenique V, Dubcovsky J.  (2004) The wheat VRN2 gene is a flowering repressor down-regulated by vernalization. Science 303:1640-1644. Impact Factor 33.6
  • Bennetzen JL, Coleman C, Liu R, Ma J, Ramakrishna W (2004) Consistent over-estimation of gene number in complex plant genomes.  Current Opinion in Plant Biology 7:732-736. Impact Factor 7.9
  • Swigonova Z, Lai JS, Ma JX, Ramakrishna W, Llaca M, Bennetzen JL, Messing J. (2004) On the tetraploid origin of the maize genome. Comparative Functional Genomics 5:281-284. Impact Factor 2
  • Ramakrishna W, Deng Z, Ding CK, Handa AK, Ozminkowski RH Jr. (2003) A novel small heat shock protein gene, vis1, contributes to pectin depolymerization and juice viscosity in tomato fruit. Plant Physiology, 131:725-735. Impact Factor 8
  • Ramakrishna W, Dubcovsky J, Park Y-J, Busso C, Emberton J, SanMiguel P, Bennetzen JL.              (2002) Different types and rates of genome evolution detected by comparative sequence analysis of orthologous segments from four cereal genomes.  Genetics 162:1389-1400. Impact Factor 6
  • Ramakrishna W, Emberton J, Ogden M, SanMiguel P, Bennetzen JL. (2002). Structural analysis of the maize Rp1 complex uncovers numerous sites and unexpected mechanisms of local rearrangement.  Plant Cell 4:3213-3223. Impact Factor 10.5
  • Ramakrishna W, Emberton J, SanMiguel P, Ogden M, Llaca V, Messing J, Bennetzen JL. (2002) Comparative sequence analysis of the sorghum Rph region and the maize Rp1 resistance gene complex. Plant Physiology 130:1728-1738. Impact Factor 8
  • Bennetzen JL, Ramakrishna W. (2002) Numerous small rearrangements of gene content, order and orientation differentiate grass genomes.  Plant Mol Biol 48:821-827. Impact Factor 4.3
  • Yan L, Echenique V, Busso C, SanMiguel P, Ramakrishna W, Bennetzen JL, Harrington S, Dubcovsky J.  (2002) Cereal genes similar to Snf2 define a new subfamily that includes human and mouse genes. Molecular Genetics Genomics 268:488-499. Impact Factor 2.7
  • Stam M, Belele C, Ramakrishna W, Dorweiler JE, Bennetzen JL, Chandler VL. (2002) The regulatory regions required for B' paramutation and expression are located far upstream of the maize b1 transcribed sequences. Genetics 162:917-930. Impact Factor 6
  • Bennetzen JL, Ramakrishna W.  (2002)Exceptional haplotype variation in maize. Proceedings of the National Academy of Sciences USA 99:9093-9095. Impact Factor 9.7

Dr. Monisha Dhiman, Associate Professor
Peer-reviewed research publications

  • Zago M, Hosakote Y, Dhiman M, Piñeyro M, Parodi-Talice A, Basombrio M, Robello C, Garg N (2016) TcI isolates of Trypanosoma cruzi exploit antioxidant network for enhanced intracellular survival in macrophages and virulence in mice. Infection and Immunity, 84(6):1842-56. Impact Factor 3.6.  
  • Gill I, Kaur S, Kaur N, Dhiman M, Mantha AK (2017) Phytochemical Ginkgolide B attenuates Aβ(1-42)- induced oxidative damage and altered cellular responses in human neuroblastoma SH-SY5Y cells. Journal of Alzheimer’s Disease. doi: 10.3233/JAD-161086. Impact Factor 3.7.
  • Dhiman, M. and Garg, N. J. (2014) P47phox-/- mice are compromised in expansion and activation of CD8+ T          cells and susceptible to Trypanosoma cruzi  infection. PLOS Pathogens. Impact Factor 8.4.
  • Kaur, N. Dhiman, M. Perez-Polo, J.R. and Mantha, A.K. (2015) Ginkgolide B Revamps Neuroprotective Role of APE1 and Mitochondrial OXPHOS Against Aβ(25-35)-Induced Neurotoxicity in Human Neuroblastoma Cells. Journal of Neuroscience Research. 9 Feb: DOI: 10.1002/jnr.23565. Impact Factor 2.7
  • Thakur, S. Sarkar, B. Cholia, R.P. Gautam, N. Dhiman, M. Mantha, A.K. (2014). APE1/Ref-1 as an Emerging Therapeutic Target for Various Human Diseases: Phytochemical Modulation of its Functions. Experimental Molecular Medicine. Jul 18(46): e106. doi: 10.1038/emm.2014.42. Impact Factor 2.46
  • Thakur, S. Dhiman, M. Tell, G. and Mantha, A.K. (2015). A Review on Protein-Protein Interaction Network of APE1/Ref-1 and its Associated Biological Functions. Cell Biochemistry & Function. 33(3):101-12. doi: 10.1002/cbf.3100. Epub 2015 Mar 19.Impact Factor 2.134
  • Dhiman, M. Wan, X. Popov, V. L. Vargas, G. Garg, N. J. (2013) MnSODtg Mice Control Myocardial Inflammatory and Oxidative Stress and Remodeling Responses Elicited in Chronic Chagas Disease. Journal of American Heart Association 2 (5): e000302 Impact Factor 5.8
  • Dhiman, M. Coronad, Y.A. Vallejo, C.K. Petersen, J.R. Ejilemele, A. Nuñez, S. Zago, M.P. Spratt, H. Garg, N.J. (2013) Innate immune responses and antioxidant/oxidant imbalance are major determinants of human Chagas disease. PLoS Negl Trop Dis. Aug; 7(8):e2364.  Impact Factor 4.8
  • Gupta, S. Wan, X. Zago, M. P.  Martinez, Sellers V.  Silva, T. S. Assiah, D. Dhiman, M. Nuñez, S.  Petersen, J. R. Vázquez-Chagoyán, J. Estrada-Franco, J. G. Garg, N. J. (2013) Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. PLoS Negl Trop Dis, 7(1):e2018 Impact Factor 4.8                                                       
  • Eaves-Pyles, T. Patel, J. Arigi, E. Cong, Y. Cao, A. Garg, N. Dhiman, M. Pyles, R.B. Arulanandam, B. Miller, A.L. Popov, V.L. Soong. L. Carlson, E. Coletta, C. Szabo, C. Almeida, I. (2013). Immunomodulatory and anti-bacterial effects of cystatin-9 against F. tularensis. Mol Med. Aug 2. doi: 10.2119/molmed.2013.00081.Impact Factor4.5
  • Gupta S, Wan X., Zago M. P,  Martinez Sellers V,  Silva T S, Assiah D, Dhiman M, Nuñez S, Petersen   J R, Vázquez-Chagoyán J, Estrada-Franco J G, Garg N J. (2013) Antigenicity and diagnostic potential of vaccine candidates in human Chagas disease. PLoS Negl Trop Dis 2013; 7(1):e2018 Impact Factor 4.8                 
  • Mantha AK, Dhiman M, Taglialatela G, Perez-Polo RJ, Mitra S. (2012) Proteomic study of amyloid beta (25-35) peptide exposure to neuronal cells: Impact on APE1/Ref-1's protein-protein interaction. J Neurosci Res. Jun; 90(6):1230-9 Impact Factor 2.99
  • Dhiman M, Paola M. Zago, Sonia Nunez, Federico Nunez-Burgio and Nisha Jain Garg. (2012) Cardiac-oxidized Antigens Are Targets of Immune Recognition by Antibodies and Potential Molecular Determinants in Chagas Disease Pathogenesis. PLoS One 7; (1):e28449.   Impact Factor  3.8
  • Dhiman M and Garg NJ. (2011) Inhibition of NADPH-oxidase Attenuates T cruzi-induced Cardiac Pathology.  Journal of Pathology (2011) Dec; 225(4):583-96.  Impact Factor 7.2    
  •  Jose´ E. Aparicio-Burgos, Laucel Ochoa-Garcıa, Jose´ Antonio Zepeda-Escobar, Shivali Gupta, Monisha Dhiman, Jose´ Simon Martınez, Roberto Montes de Oca-Jimenez, Margarita Val Arreola, Alberto Barbabosa-Pliego, Juan C. Vazquez-Chagoyan, Nisha Jain Garg (2011). Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs. PLoS Negl Trop Dis  5(5): 1-10. Impact Factor 4.8
  • Wen J-J, Gupta S, Guan Z, Dhiman M, Condon D, Lui CY, Garg NJ (2010). Phenyl-alpha-tert-butyl-nitrone and benzonidazole treatment controlled the mitochondrial oxidative stress and evolution of cardiomyopathy in chronic chagasic Rats. J Amer College Cardiology. 2010, 55(22):2499-508.   Impact Factor 16
  • Dhiman M, Jasmine Pando, Estrada-Franco JG, Aguilar FR, Corzo SB, Perez GM, Sandoval RG, Garg NJ. (2009) Increased myleoperoxidase activity and protein nitration are indicators of inflammation in chagasic patients. Clinical and Vaccine Immunology, 16(5):  660-666. Impact Factor3.08
  • Dhiman M, Nakayasu ES, Hosakote YM, Reynolds BK, Wen JJ, Almeida IC and Garg NJ. (2008) Enhanced Nitrosative Stress during Trypanosoma cruzi Infection Causes Nitrotyrosine Modification of Host Proteins. Implications in Chaga’s disease.  Am J Pathol.  173 (3): 728-740. Impact Factor 6.34
  • Wen J-J*, Dhiman M*, and Garg NJ. (2008) Tissue-specific oxidative imbalance and mitochondrial dysfunction during Trypanosoma cruzi infection in mice. Microbes Infect.  10 1201-1209. *Equal Contribution Impact Factor 3.0                                                          
  • Deep G, Dhiman M, Mendiz E, Rao AR and Kale RK. (2005) Chemo preventive Effects of Mustard Seeds in Chemically Induced Fore stomach and Uterine Cervix Tumors in Murine Model System. Human & Experimental Toxicology. 24 (6): 303-312.                  Impact Factor 2.78 
  • Deep G, Dhiman M, Rao AR and Kale RK. (2005). Chemopreventive Potential of Triphala on Benzo(a)Pyrene Induced Forestomach Tumorigenesis in Murine Tumor Model System. Journal of Experimental & Clinical Cancer Research.  24 (4) 555-563. Impact Factor 4.43  

Dr. Dr. Malkhey Verma, Associate Professor
Peer-reviewed research publications

  • Sawaya MR, Verma M, Balendiran V, Rath NP, Cascio D, Balendiran GK (2016). Characterization of WY 14,643 and its Complex with Aldose Reductase. Scientific Reports 6:34394.
  • Mondeel TDGA, Rehman S, Zhang Y, Verma M, Dürre P, Barberis M, Westerhoff HV (2016). Maps for when the living gets tough: Maneuvering through a hostile energy landscape. IFAC-PapersOnLine 49-26: 364–370.
  • Bragg S, Verma M, Moser RJ and Balendiran GK (2015). Kinetics of the Solid State Pyrolysis of Gellan Gum and Paper Pulp. Journal of Biobased Materials and Bioenergy Vol. 9, 1-8.
  • Balendiran K, Ganesaratnam, Rajendran Pandian, J., Drake, Evin, Vinayak Anubhav, Verma M, Cascio Duilio (2014). B-factor Analysis and Conformational Rearrangement of Aldose Reductase. Current Proteomics 11(3): 151-160.
  • Ettore Murabito, Malkhey Verma, Martijn Bekker, Domenico Bellomo, Hans V. Westerhoff, Bas Teusink, Ralf Steuer (2014). Monte-Carlo Modeling of the Central Carbon Metabolism of Lactococcus lactis: Insights into Metabolic Regulation. PLoS ONE 9(9): e106453
  • M Verma, EG Karimiani, RJ Byers, S Rehman, HV Westerhoff, PJR Day (2013). Mathematical modelling of miRNA mediated BCR-ABL protein regulation in chronic myeloid leukaemia vis-a-vis therapeutic strategies. Integrative Biology 5(3): 543-54.
  • M Verma, M Zakhartsev, M Reuss, HV Westerhoff (2012). ‘Domino’ systems biology and the ‘A’of ATP. Biochimica et Biophysica Acta (BBA)-Bioenergetics 1827(1): 19-29.
  • GK Balendiran, MR Sawaya, FP Schwarz, G Ponniah, R Cuckovich, M Verma, D Cascio (2011). The role of Cys-298 in aldose reductase function. Journal of Biological Chemistry 286 (8): 6336-6344
  • HV Westerhoff, M Verma, M Nardelli, M Adamczyk, K van Eunen, E Simeonidis (2010). Systems Analysis of Metabolism. Biochemical Society transactions 38 (part 5): 1189-1196.
  • HV Westerhoff, C Winder, H Messiha, E Simeonidis, M Adamczyk, M Verma, FJ (2009). Systems biology: the elements and principles of life. FEBS letters 583 (24): 3882-3890.
  • M Verma, HJ Martin, W Haq, TR O'Connor, E Maser, GK Balendiran (2008). Inhibiting wild-type and C299S mutant AKR1B10; a homologue of aldose reductase upregulated in cancers. European journal of pharmacology 584(2): 213-221.
  • GK Balendiran, M Verma, E Perry (2007). Chemistory of fibrates. Current Chemical Biology 1(3): 311-316
  • M Verma, S Rawool, PJ Bhat, KV Venkatesh (2006). Biological significance of autoregulation through steady state analysis of genetic networks. Biosystems 84(1): 39-48
  • M Verma, PJ Bhat, KV Venkatesh (2005). Steady-state analysis of glucose repression reveals hierarchical expression of proteins under Mig1p control in Saccharomyces cerevisiae. Biochemical Journal 388 (Pt 3): 843-849.
  • A Ruhela, M Verma, JS Edwards, PJ Bhat, S Bhartiya, KV Venkatesh (2004). Autoregulation of regulatory proteins is key for dynamic operation of GAL switch in Saccharomyces cerevisiae. FEBS Letter 576(1-2): 119-126.
  • M Verma, PJ Bhat, KV Venkatesh (2004). Expression of GAL genes in a mutant strain of Saccharomyces cerevisiae lacking GAL80: quantitative model and experimental verification. Biotechnology and Applied Biochemistry 39(1): 89-97.
  • M Verma, PJ Bhat, S Bhartiya, KV Venkatesh (2004). Steady state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae. European Journal of Biochemistry 271 (20): 4064-4074.
  • M Verma, PJ Bhat, KV Venkatesh (2003). Quantitative analysis of GAL genetic switch of Saccharomyces cerevisiae reveals that nucleocytoplasmic shuttling of Gal80p results in a highly sensitive response to galactose. Journal of Biological Chemistry 278 (49): 48764-48769.
  • E Murabito, R Colombo, Cn Wu, M Verma, S Rehman, J Snoep, S-L Peng, N Guan, HV Westerhoff (2015). SupraBiology 2014: Promoting UK-China collaboration on Systems Biology and High Performance Computing. Quantitative Biology: 2015: eScholarID: 259998. DOI:10.1007/s4048401500399

Dr. Somesh Baranwal, Assistant Professor
Peer-reviewed research publications

  • D Wang, NG Naydenov, A Feygin, S Baranwal, JF Kuemmerle, AI Ivanov. Actin-depolymerizing factor and cofilin-1 have unique and overlapping functions in regulating intestinal epithelial junctions and mucosal inflammation research article submitted to American Journal of Pathology. Impact Factor 4.59
  • S Lechuga, S Baranwal, AI Ivanov. (2015) Actin interacting protein 1 controls assembly and permeability of intestinal epithelial apical junctions. Am J Physiol Gastrointest. Liver Physiol. 308 (9):G745-56. Impact Factor 3.72
  • C Sharon, S Baranwal, NJ Patel, D R-A, WM Pandak, APN Majumdar, G Krystal, BB Patel. (2015) Inhibition of Insulin-like Growth Factor Receptor/AKT/Mammalian Target of Rapamycin Axis Targets Colorectal Cancer Stem Cells by Attenuating Mevalonate-Isoprenoid Pathway in vitro and in vivo. Oncotarget. 6(17):15332-47. Impact Factor 6.63
  • S Lechuga*, S Baranwal*, C Li , NG Naydenov, JF. Kuemmerle, V Dugina , C Chaponnier, AI Ivanov (2014) Loss of  γ-cytoplasmic actin triggers myofibroblast transition of human epithelial Cell (* denotes equal contribution) Mol Biol Cell.  25(20):3133-46 Impact Factor 5.86
  • J Patel*, S Baranwal*, I Love, N Patel, S Grossman, BB Patel. Inhibition of C-terminal Binding Protein attenuates Transcription Factor 4 signaling to selectively target Colon Cancer Stem Cells. (*denotes equal contribution) Cell Cycle. 2014 Nov 15; 13(22):3506-18. Impact Factor 5.006
  • Patel, NJ*, K Rajesh, Al-Horani R*, S Baranwal, Patel J, Desai UR, Patel, B (2014) Synthetic, Non-Saccharide Glycosaminoglycan Mimetics Selectively Target Colon Cancer Stem Cells (*denotes equal contribution) ACS Chem Biol. 9(8):1826-33. Impact Factor 5.44
  • Patel J*, S Baranwal*, IM Love, NJ Patel, A Raqhibana, C Sharon, BB Patel (2014) C-Terminal Binding Protein is an important therapeutic target for Colorectal Cancer to Selectively aim at Cancer Stem Cells by regulating β-Catenin signaling., Gastroenterology 146 (5), S-516-S-517 (Abstract). Impact Factor 16.716
  • Patel NJ*, R Karuturi*, RA Al-Horani*, S Baranwal, J Patel, UR Desai, BB Patel (2014) Synthetic, non-saccharide glycosaminoglycan mimetics selectively target colon cancer stem cells  Gastroenterology 146 (5), S-84-S-85  (Abstract). Impact Factor 16.716
  • S Chetna, S Baranwal, J Patel, NJ Patel, BB Patel.(2014) Inhibition of Insulin-Like Growth Factor Receptor Activity Attenuates Colon Cancer Stem Cells Growth and Self-Renewal by Targeting Mevlonate-Isoprenoid Metabolism, Gastroenterology 146 (5), S-692 (Abstract) Impact Factor 16.716
  • Dong S, S Baranwal, M. Maziveyi, S. Alahari (2013) Nischarin-integrin interaction and downstream signaling regulate mouse mammary development and tumorigenesis Mol. Biol. Cell, December 15, vol. 24 # 888 (Abstract) Impact Factor 4.54
  • NG Naydenov*, S Baranwal*, S Khan, A Feygin, P Gupta, AI Ivanov (2013) Novel mechanism of cytokine-induced disruption of epithelial barriers: janus kinase and protein kinase D dependent down regulation of junction protein expression Tissue Barriers. (*denotes equal contribution) Oct 1;1(4):e25231
  • P Jain, S Baranwal, AP Struckhoff, RA Worthylake, SK Alahari  (2013) LKB1 interacts with Nischarin to regulate breast tumor growth and metastasis. J Biol Chem.  288(22):15495-509. Impact Factor 4.77
  • S Baranwal, NG Naydenov, G Harris, V Dugina, KG Morgan, C Chaponnier, AI Ivanov (2012) Non-redundant roles of cytoplasmic beta- and gamma-actin isoforms in regulation of epithelial apical junctions Mol Biol Cell. 23(18):3542-53. Impact Factor 5.86
  • NG Naydenov, B Brown, G Harris, MR Dohn, VM Morales, S Baranwal, AB Reynolds, AI Ivanov (2012) A membrane fusion protein alpha-SNAP is a novel regulator of epithelial apical junctions   PLoS One. 7(4):e34320 Impact Factor 4.11
  • S Baranwal, Wang Y, Rathinam R, Lee J, Jin L, McGoey R, Pylayeva Y, Giancotti F, Blobe GC, Alahari SK. (2011) Molecular characterization of the tumor-suppressive function of Nischarin in breast cancer.  J National Cancer Inst. 103 (20): 1513-28 Impact Factor 14.69
  • P Jain, S Baranwal, SK Alahari, (2011) Tumor suppressor LKB1 cooperates with the integrin-binding protein Nischarin to inhibit breast cancer Cancer Research. 71(8 Supplement):2196-2196. (Abstract) Impact Factor 9.28
  • Yanfang W*, Shenouda S*, Somesh Baranwal*, Rathinam, R. Jain P., Hazari S, Dash S, Alahari SK. (2011) Integrins alpha-5 and alpha-6 regulate cell cycle by modulating the Chk1 and Rb/E2F pathways to affect breast cancer metastasis. Molecular Cancer. 10:84. (*Denotes equal contribution) Impact Factor 5.4
  • S Baranwal, Alahari SK (2011) Rho GTPase Effector Functions in Tumor Cell Invasion and Metastasis. Current Drug Targets.  12(8):1194-201. Impact Factor 3.1
  • S Baranwal, Alahari SK. (2010) miRNA control of tumor cells invasion and metastasis. Int J Cancer. 126 (6): 1283-90. Impact Factor 5.44
  • S Baranwal, Walch A, Lakins J, Weaver VM and Alahari SK. Nischarin over expression inhibits metastasis by restoring epithelial markers in metastatic breast cancer cells Mol. Biol. Cell 19 (suppl), #. 1436/B655 (Abstract) Impact Factor 5.979
  • Wang, Y, Lavezzi, T, Walch Amelia, Milosavljevic T, S Baranwal, S.K.  Alahari. December, (2007) “MicroRNA Profiling of Human Breast Cancer Cell Line MDA-MB-231 (sub-line 4175) and MCF10A. Mol. Biol. Cell 19 (suppl), #. 1436/B655 (Abstract) Impact Factor 5.57
  • Fong KP, Pacheco CM, Otis LL, S Baranwal, Kieba IR, Harrison G, Hersh EV, Boesze-Battaglia K, Lally ET. (2006) Actinobacillus actinomycetemcomitans leukotoxin requires lipid micro domains for target cell cytotoxicity.  Cellular Microbiology 8(11): 1753-67. Impact Factor 5.62.

Dr. Shashank Kumar, Assistant Professor
Peer-reviewed research publications

  • Bais S S, Lawrence K, Kumar S, Lawrence R (2015). Eichhornia crassipes (Mart.) Solm: A biotic resource for waste water treatment. World J Pharmaceut Sci. 3:1
  • Sanjay S S, Pandey A C, Kumar  S, Pandey A K. (2014).Membrane protective efficacy of ZnO nanoparticles,” OP Transactions on Nano-technology. 1:1, 21-29
  • Kumar S and Pandey AK. (2014). Medicinal attributes of S. xanthocarpum fruit consumed by several tribal communities as food: An antioxidant, anticancer and anti HIV perspective,” BMC Comp Alt Med, 14:112
  • Kumar S, Pandey S, Pandey A. K. (2014). In vitro antibacterial, antioxidant, cytotoxic activities of Parthenium hysterophorus and characterization of extracts by LC MS analysis. BioMed Research International (Formerly titled Journal of Biomedicine and Biotechnology). Article ID 495154
  • Kumar S, and Pandey A. K. (2014). Preliminary evaluation of biological activities and phytochemical analysis of Syngonium podophyllum leaf. National Academy Science Letters, 38:2, 143-146
  • Kumar S, Kumar R, Diwedi A, and Pandey A. K. (2014). In vitro antioxidant, antibacterial, cytotoxic activity and in vivo efficacy of Syngonium podophyllum and Eichhornia crassipes leaf extracts on isoniazid induced oxidative stress and hepatic markers. BioMed Res Int. 2014: Article ID 459452
  • Sharma A K, Kumar S and Pandey A K. (2014). Cell cycle inhibitory activity of Piper longum against A549 cell line and its protective effect against metal-induced toxicity in rats. Ind J Biochem Biophys. 2014, 51, October
  • Sharma A K, Kumar S and Pandey A K. (2014). Ferric reducing, antiradical and cytotoxic activites of Tinospora cordifolia stem extract. Biochem Anal Biochem. 3:2
  • Mishra A, Kumar S and Pandey AK. (2013). Scientific validation of the medicinal efficacy of Tinospora cordifolia,” Scientific World J, 2013, Article ID 292934.
  • Kumar S, Chashoo G, Saxena AK and Pandey AK, (2013). Parthenium hysterophorus: a probable source of anticancer, antioxidant and anti-HIV agent. BioMed Res Int., 2013: Article ID 810734
  • Mishra A, Sharma AK, Kumar S, Saxena AK and Pandey AK. (2013). Bauhinia variegata leaf extracts exhibit considerable antibacterial, antioxidant and anticancer activities. BioMed Res Int, 2013: Article ID 915436, 10 pages.
  • Kumar S, Gupta AK and Pandey AK. (2013) Calotropis procera root extract has capability to combat free radical mediated damage. ISRN Pharmacol, 2013: Article ID 691372
  • Kumar S, Mishra A, and Pandey AK. (2013) Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Comp Alt Med, 13:120
  • Kumar S, and Pandey AK.. (2013). Phenolic content, reducing power and membrane protective activities of Solanum xanthocarpum root extracts. Vegetos- An Int J Plant Res, 26, 301-307
  • Kumar S, Sharma UK, Sharma AK and Pandey AK. (2012). Protective efficacy of Solanum xanthocarpum root extracts against free radical damage: phytochemical analysis and antioxidant effect.  Cell Mol Biol, 58, 171-178.
  • Kumar S, and Pandey AK. (2012.) Antioxidant, lipo-protective and antibacterial activities of phytoconstituents present in Solanum xanthocarpum root. Int Rev Biophysical Chem, 3, 42-47
  • Mishra A, Kumar S, Bhargava, A, Sharma, B, and Pandey, A.K. (2011). Studies on in vitro antioxidant and anti staphylococcal activities of some important medicinal plants, Cell Mol Biol, 57, 16-25
  • Pandey A K, Mishra A, Kumar S, and Chandra, A. (2010). Therapeutic Potential of C. zeylanicum extracts: An antifungal and antioxidant perspective, Int J Biol Med Res. 1(4): 228-233
  • Kumar S and Pandey AK. (2015). Free radicals, from oxidative stress to disease and their mitigation by herbals, British J Med Med Res. 7 (6), 438-457
  • Kumar S and Pandey AK. (2014). Pharmacological activities of some common Indian weeds: A review, Mintage J Pharmaceut Med Sci, 3, 12-17
  • Kumar S, Gupta R, Kaleem AM, Pandey AK. (2014). Mitigation of pain and anesthetic drugs, OA Anaesthetics, 2 (1): 2
  • Pandey A K, and Kumar S. (2014) Perspective on plant products as antimicrobials agents: A review, Pharmacologia4: 469-480
  • Kumar S (2015). MicroRNAs an evolving prognostic, diagnostic and therapeutic target in prostate cancer. World J Pharmaceut Sci. 3:3, 387-389
  • Kumar R, Diwedi A, Kumar S and Pandey A. K. (2014) Effect of Eichhornia crassipes leaf aqueous extract on isoniazid induced oxidative stress and hepatic markers in albino wistar rats. In: Proceedings of All India Seminar on Recent Advances in Biotechnology and Human Health Care (March 2014). Editors R. Shrivastava, and B. Modhera, 1-7
  • Kumar S, and Pandey, AK, (2013) Phytochemicals analysis, cytotoxic and radical scavenging activities of Parthenium hysterophorus. In: Proceedings of Intl Conf on health, environment and industrial biotechnology (ICHEIB BioSangam 2013). Editor S. Sharma, McGraw Hill Education (India) Pvt Ltd., New Delhi. pp 271-274. ISBN (13): 978-9-33-290137-7

 Review Articles Published

  • Kumar S, Ahamad MK, Waseem M, Pandey AK (2015). Drug targets for cancer treatment: An overview, Med Chem. 5:3, 115-123
  • Kumar S and Pandey AK. (2013). Chemistry and Biological Activities of Flavonoids: An Overview. Scientific World J, 2013,Article ID162750.
  • Kumar S (2014). Emerging trends in pharmaceutical sciences: An introduction. World J Pharmaceut Sci. 2:12, 1592-1593

Dr. Pramod Kushawaha, Assistant Professor
Peer-reviewed research publications

  • Tripathi CP,  Kushawaha PK, Sangwan RS, Mandal C, Misra Bhattacharya S, Dube A (2016): Withania somnifera chemotype NMITLI 101R significantly increases the efficacy of antileishmanial drugs by generating strong IFN-γ and IL-12 mediated immune responses in Leishmania donovani infected hamsters. Phytomedicine. 24:87-95. Impact Factor-3.5
  • Kushawaha PK, Gupta R, Sundar S, Sahasrabuddhe AA and Dube A (2011). Elongation Factor-2- a Th1 stimulatory protein of Leishmania donovani generates strong IFN-γ and IL-12 response in cured Leishmania-infected patients/hamsters and protects hamsters against Leishmania challenge. J Immunol, 187: 6417–6427. Impact Factor-4.92
  • Kushawaha PK, Gupta R,  Tripathi CD,  Sundar S,  Dube A (2012): Evaluation of Leishmania donovani Protein disulfide isomerase as a potential immunogenic protein / vaccine candidate against visceral leishmaniasis. PLoS One, Volume 7/Issue 4/ e356770. Impact Factor-3.23
  • Kushawaha PK, Gupta R, Tripathi CD, Khare P, Jaiswal AK, Sundar S, Dube A (2012): Leishmania donovani Triose Phosphate Isomerase: a potential vaccine target against Visceral Leishmaniasis. PLoS One, Volume 7 | Issue 9 | e45766. Impact Factor-3.23
  • Gupta R *, Kushawaha PK *, Samant M, Jaiswal AK, Baharia AK, Dube A (2012). Treatment of Leishmania donovani- infected hamsters with miltefosine: analysis of cytokine mRNA expression by real- time PCR, lymphoproliferation, nitrite production and antibody response.  J Antimicrob Chemother. 2012 Feb;67(2):440-3.* Equally contributed. Impact Factor-5.33
  • Gupta R, Kushawaha PK, Tripathi CD, Sundar S, Dube A (2012): A novel recombinant Leishmania donovani p45-a partial coding region of methionine aminopeptidase protein generates protective immunity by inducing Th1 stimulatory response against experimental visceral Leishmaniasis. International Journal of Parasitology, May 1;42(5):429-35. Impact Factor-3.83
  • Samant M, Gupta R, Kumari S, Misra P, Khare P, Kushawaha PK,  Sahashrabuddhe AA, Dube A (2009).Immunization with the DNA Encoding N-terminal domain of Proteophosphoglycan (PPG) of Leishmania donovani generates Th-1 type immuno-protective response against Experimental Visceral Leishmaniasis. J Immunol Jul 1; 183(1):470-9. Impact Factor-4.92
  • Misra P, Khaliq T, Dixit A, SenGupta S, Samant M, Kumari S, Kumar A, Kushawaha PK, Majumder HK, Saxena AK, Narender T, Dube A (2008). Antileishmanial activity mediated by apoptosis and structure – based target study of Peganine hydrochloride, an approach for rational drug design. J Antimicrob chemother. Nov, 62 (5):998-1002. Impact Factor-5.33
  • Gupta R, Kumar V, Kushawaha PK, Tripathi CP, Joshi S, Sahasrabuddhe AA, Mitra K, Sundar S, Siddiqui I, Dube A (2014): Characterization of Glycolytic Enzymes - rAldolase and rEnolase of Leishmania donovani, Identified as Th1 Stimulatory Proteins, for Their Immunogenicity and Immunoprophylactic Efficacies against Experimental Visceral Leishmaniasis. PLoS One, 9(1): e86073. Impact Factor-3.23
  • Tripathi CD, Gupta R, Kushawaha PK, Mandal C, Misra Bhattacharya S, Dube A (2014). Efficacy of Withania somnifera chemotypes NMITLI - 101R, 118R and Withaferin A against experimental visceral leishmaniasis.  Parasite Immunology, 36, 253–265. Impact Factor-2.2
  • Jaiswal, Khare P, Joshi S, Kushawaha PK, Sundar S, Dube A (2014): Th1 stimulatory proteins of Leishmania donovani: Comparative cellular and protective responses of rTriose phosphate isomerase, rProtein disulfide isomerase and rElongation factor-2 in combination with rHSP70 against visceral leishmaniasis. PLoS One. Sep 30;9(9):e108556. Impact Factor-3.23

Dr. Manju Jain, Assistant Professor
Peer-reviewed research publications

  • Khandelwal A, Malhotra A, Jain M, Vasquez KM, Jain A. (2017). The emerging role of long non-coding RNA in gallbladder cancer pathogenesis. Biochimie 132:152-160
  • D Lomada, M Jain*, M Reddy, R Kang, J DiGiovanni, E Richie, (2016). Stat3 Signaling Promotes Survival And Maintenance Of Medullary Thymic Epithelial Cells” mTEC development , Plos Genetics. * Equal first co-author, Impact Factor 7.53
  • M Jain and R Madhubala, (2008). Characterization and localization of ORFF gene from the LD1 locus of Leishmania donovani. Gene, Jun 15; 416 (1-2): 1-10, Impact Factor 2.18
  • B Sukumaran, M Jain, PJ Myler, KD Stuart and R Madhubala, (2007). Creation of Tetracycline Regulatable Knockouts of ORFF Gene from the LD1 Locus of Leishmania donovani. American Journal of Biochemistry and Biotechnology 3(4): 207-215
  • M Jain, VS Dole, PJ Myler, KD Stuart and Ra Madhubala, (2007). Role of Biopterin Transporter (BT1) Gene on Growth and Infectivity of Leishmania. American Journal of Biochemistry and Biotechnology 3(4): 199-206
  • P Tewary, M Jain, MH Sahani, S Saxena and R Madhubala, (2005). A heterologous prime boost vaccination regime using ORFF DNA and recombinant ORFF protein confers protective immunity against experimental visceral leishmaniasis. J. Infec. Diseases 191: 2130-2137, Impact Factor 6.2

List of the Research Projects Operational in the Centre

  1. Dhiman, M. Chemotherapeutic Drug Induced Cardiomyocyte Toxicity: Evaluation of Ethno-Botanical Plants to Minimize the Cardiac Damage. Funded by Dept. of Science and Technology (DST), New Delhi. PI (22 Lakhs) (2013-17)
  2. Dhiman, M. Mitochondrial Oxidative DNA Damage-Repair in Alzheimer’s disease: AP-endonuclease (APE1/Ref-1) as a Potential Therapeutic Target. Funded by DST, New Delhi. Co-investigator (46 Lakhs) (2014-17).
  3. Somesh, B. Role of Integrin binding protein, kindlin in colon cancer progression. Funded by Dept. of Biotechnology (DBT). PI (88 Lakhs) (2015-2020)
  4. Pramod, K. Functional characterization of the guanylate binding proteins- interferon- γ- inducible 65- KD GTPases in host immune response to Leishmania pathogenesis. Funded by DST, New Delhi. PI (35 Lakhs) (2014-2019)
  5. Somesh B. Role of Nischarin in regulation of intestinal apical epithelial junction. Funded by DST. PI (50 Lakhs) (2017-2020)
  6. Somesh B. Identification and characterization of functional significance of gastric cancer stem cells. Funded by UGC-FRP Scheme. PI (10 Lakhs) (2017-2019)
  7. Shashank K. Effect of Withania somnifera phytoconstituents on miRNA expression profile in breast cells: Therapeutic and Cytoprotective role.  Funded by DST, New Delhi. PI             (50 Lakhs) (2017-2021)
  8. Shashank K. Identification and validation of novel notch signaling pathway inhibitors as potential anticancer agents of natural origin. Funded by UGC. PI (10 Lakhs) (2017-2019)
  9. Manju J.  Investigating the role of thymus in mediating host immune- modulation in Visceral Leishmaniasis. Funded by DST-SERB Fast Track. PI (30.3 Lakhs) (2014-2018)
  10. Ramakrishna W.  Identification of best combination of plant growth promoting bacteria and arbuscular mycorrhizae for enhancing yield and nutrient content of wheat and dissecting associated biological pathways using proteomics and metabolomics. Funded by DST-SERB. PI (38.2 Lakhs) (2017-2020)

Schedule for the PhD student presentations in current semester